Journal: Scientific Reports

Article Title: Production and characterization of rhamnolipids by Pseudomonas aeruginosa isolated in the Amazon region, and potential antiviral, antitumor, and antimicrobial activity

doi: 10.1038/s41598-024-54828-w

Figure Lengend Snippet: Minimum inhibitory concentration (MIC, µg/mL) of the biosurfactant produced by P. aeruginosa BM02 against pathogenic microbial strains.

Article Snippet: It was assessed by the microdilution method according to the recommendations of the National Committee for Clinical Laboratory Standards, against Staphylococcus aureus (ATCC 25,923), Escherichia coli (ATCC 25,922), Enterococcus faecium (ATCC 6569) and Salmonella choleraesuis (ATCC 10,708), and the strains Pseudomonas aeruginosa (INCQS 2742) and Candida albicans (INCQS 72,000), provided by the National Institute for Quality Control in Health, Fundação Oswaldo Cruz, Brazil.

Techniques: Concentration Assay, Produced

Journal: Saudi Journal of Biological Sciences

Article Title: Propolis characterization and antimicrobial activities against Staphylococcus aureus and Candida albicans : A review

doi: 10.1016/j.sjbs.2021.11.063

Figure Lengend Snippet: Chemical characterization of different types of propolis, geographic distribution, botanical origin, and biological activities.

Article Snippet: Similarly, ethanol extracts of Polish propolis (EEPP) have shown antibacterial activity against S. aureus (ATCC 25,923 and ATCC 29213) with MIC values ranging from 128 to 512 μg/mL, and weak bactericidal activity with MBC values from 512 up to 4096 μg/mL.

Techniques: Activity Assay

Journal: Cancer science

Article Title: Balance between SIRT1 and DBC1 expression is lost in breast cancer.

doi: 10.1111/j.1349-7006.2010.01573.x

Figure Lengend Snippet: Fig. 1. Immunohistochemical scoring of SIRT1 and DBC1 expression. Left column, SIRT1 staining intensity from negative to 3+ (top to bottom); right column, DBC1 staining intensity from negative to 3+ (top to bottom) (· 400).

Article Snippet: The antibodies used for western blotting were: SIRT1 (E104; Epitomics, Burlingame, CA, USA), DBC1 (antisera against 2–25 and 900–923 amino acids), and GADPH (SC-25778; Santa Cruz Biotechnology, Santa Cruz, CA, USA).

Techniques: Immunohistochemical staining, Expressing, Staining

Journal: Cancer science

Article Title: Balance between SIRT1 and DBC1 expression is lost in breast cancer.

doi: 10.1111/j.1349-7006.2010.01573.x

Figure Lengend Snippet: Fig. 2. Expression of both SIRT1 and DBC1, as detected by immunohistochemistry and western blotting, was higher in tumor tissues than in normal tissues. (a) SIRT1 and DBC1 expression, as demonstrated by immunohistochemistry, in normal and tumor tissue of a representative case (case no. 15) (· 400), (b) Immunohistochemistry scores of SIRT1 and DBC1 in normal and tumor tissue are shown as a scatter plot. The mean score of each group is shown as a bar, (c) The expressions of SIRT1, DBC1, and GAPDH (cases 4–6, 11, and 15) were determined by western blotting. GAPDH was used as a loading control for the analysis, (d) The densities of SIRT1 and DBC1 bands in normal and tumor tissue according to western blotting were normalized by GAPDH and are shown as a scatter plot. Means of densities for each group are shown as a bar. *P < 0.05, (e) The mRNA expressions of SIRT1, DBC1, and GAPDH were determined in six patients using RT-PCR, and (f) The mRNA expressions were normalized by GAPDH and the relative levels are shown as a scatter plot.

Article Snippet: The antibodies used for western blotting were: SIRT1 (E104; Epitomics, Burlingame, CA, USA), DBC1 (antisera against 2–25 and 900–923 amino acids), and GADPH (SC-25778; Santa Cruz Biotechnology, Santa Cruz, CA, USA).

Techniques: Expressing, Immunohistochemistry, Western Blot, Control, Reverse Transcription Polymerase Chain Reaction

Journal: Cancer science

Article Title: Balance between SIRT1 and DBC1 expression is lost in breast cancer.

doi: 10.1111/j.1349-7006.2010.01573.x

Figure Lengend Snippet: Fig. 3. Correlation between SIRT1 and DBC1 is poor in tumor tissue compared to normal tissue. The correlative value was determined by immuno- histochemistry (a) or by western blotting (b).

Article Snippet: The antibodies used for western blotting were: SIRT1 (E104; Epitomics, Burlingame, CA, USA), DBC1 (antisera against 2–25 and 900–923 amino acids), and GADPH (SC-25778; Santa Cruz Biotechnology, Santa Cruz, CA, USA).

Techniques: Immunohistochemistry, Western Blot